UV-Vis, FTIR and antioxidant study of Persea Americana (Avocado) leaf and fruit: A comparison
Resumen
Avocado (Persea americana Miller), a well-known nutritional tree crop grown in many parts of the world. The present study compares the UV-Vis and Fourier transform infrared spectroscopy (FTIR) spectrum of Avocado leaf and fruit (peel, pulp, and oil) cultivated in Ecuador; and further its antioxidant activity is evaluated against 1,1-diphenyl-2-picrylhydrazyl (DPPH•). The UV-Vis and FTIR study revealed predominant amount of flavonoids. Among the Avocado leaf and fruit studied, the DPPH• free radical scavenging assay for Avocado leaf had the highest antioxidant activity ranging from 84.46 % to 80.12% with values from 32.60-32.73 μg gallic acid equivalents per mL. It showed that the order of antioxidant activity in Avocado is leaf > peel > oil > pulp. The antioxidant activity had a positive correlation with total flavonoid content and these plant extracts (specially Avocado leaf) are useful for future antioxidant products.
El aguacate (Persea americana Miller) es una conocida fruta arbórea con un alto contenido nutricional que crece en varias partes del mundo. El presente estudio compara los espectros del UV-Vis y del espectrómetro infrarrojo con transformada de Fourier (FTIR) de la fruta y de la hoja de aguacate (cáscara, pulpa y aceite) cultivado en Ecuador y posteriormente evalúa su actividad antioxidante empleando el 1,1-difenil-2-picrilhidrazil (DPPH•). El estudio de los espctros UV-Vis y FTIR revel´o que el aguacate tiene predominantemente flavonoides. Entre la hoja y el fruto del aguacate, se comprobó mediante el ensayo DPPH• (captura de radicales libres), que la hoja tuvo una mayor actividad antioxidante, la que oscila entre 84,46% y 80,12%, con valores de 32.60-32.73 μg equivalentes de ácido gálico por mL. Se demostró que el orden de la actividad antioxidante de los extractos es: hoja de aguacate > cáscara > aceite > pulpa. La actividad antioxidante tuvo una correlación positiva con el contenido total de flavonoides y estos extractos de plantas (especialmente de las hojas del aguacate) son útiles para el desarrollo de futuros productos antioxidantes.
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