Effectiveness of Disinfection in Dental Unit Irrigation Systems Using 5% Sodium Hypochlorite and 15% Peracetic Acid
DOI:
https://doi.org/10.18537/fouc.v03.n02.a01Keywords:
sodium hypochlorite, peracetic acid, disinfection, colony-forming unit assayAbstract
Introduction: In the dental field, the disinfection
of irrigation systems is crucial for eliminating
microbial loads. Objective: To evaluate the disinfection
effectiveness in dental unit irrigation
systems using 5 % sodium hypochlorite and 15
% peracetic acid at the third-level clinic of the
Faculty of Dentistry of the Central University of
Ecuador. Materials and Methods: Experimental,
comparative, in vitro study. The sample
was selected by non-probabilistic convenience
sampling and consisted of 16 dental unit irrigation
systems, which were randomLy assigned to two
groups: Group A: 8 irrigation systems disinfected
with 5 % sodium hypochlorite. Group B: 8 irrigation
systems disinfected with 15 % peracetic acid.
The study was conducted in two phases: Pretest
phase: Collection and analysis of water samples
before disinfection. Posttest phase: Collection
and analysis of water samples after disinfection to
assess process effectiveness. A 20 mL water sample
was taken from the dental chair at the tip of the
triple syringe. Samples were collected in sterile
plastic vials and transported to the UCE Chemistry
Laboratory for processing and plating. Once results
were obtained, statistical analysis was performed.
Results: The initial aerobic mesophilic bacteria
count averaged 7 617.50 ± 13 681.79 CFU/mL. After
disinfection in both groups, no viable microorganisms
were detected. Conclusions: The disinfection process achieved 100 % effectiveness in eliminating microorganisms from dental unit irrigation systems for both disinfectants compared to the initial data
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