Determination of the antibacterial activity of extracts from medicinal plants against Enterococcus faecalis
DOI:
https://doi.org/10.18537/mskn.13.01.07Keywords:
In vitro antibacterial activity, Enterococcus faecalis ATCC 29212, Enterococcus faecalis ATCC 51299 vancomycin resistant, IC50, microdilutionAbstract
Enterococcus faecalis is a microorganism considered as an etiological agent of different infectious processes, such as: skin infections, osteomyelitis, pneumonia, urinary tract infections, endocarditis, among others, with nosocomial patients being a population more vulnerable to this bacterium. The objective of the present study was to evaluate the in vitro antibacterial activity of methanolic and chloroform extracts from the plants Peperomia galioides, Otholobium mexicanum, Zingiber officinale and Rosmarinus officinalis against Enterococcus faecalis ATCC 29212, Enterococcus faecalis ATCC 51299 (vancomycin resistant) and a clinical strain of Enterococcus faecalis. For the evaluation of this bioactivity, the microdilution methodology and cut-off points established by the CLSI (2021) for Enterococcus faecalis were used. The results obtained were expressed in IC50. The IC50 of greatest interest was obtained with the chloroform extract of Otholobium mexicanum against the clinical strain, being 2.050 μg/mL, being considered very active. The most notable IC50 of Peperomia galioides was obtained with the chloroform extract against the clinical strain with a value of 8.248 μg/mL, considering it as slightly active. For Rosmarinus officinalis, only the methanolic extract showed a slight antibacterial activity with an IC50 of 20.234 μg/mL against Enterococcus faecalis ATCC 29212. Finally, all Zingiber officinale extracts did not show any activity against the tested concentrations of the different strains of Enterococcus faecalis. The results obtained are of great relevance since they can be applied for the production of antimicrobials from natural sources that could have application within the pharmaceutical, veterinary or agricultural industry.
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Copyright (c) 2022 Oliver Ramírez-Condoy, Lourdes Jerves-Andrade, Andrea Abril, Marcela Redrován-Berrezueta
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