In vitro antifungal susceptibility evaluation of T. mentagrophytes and T. rubrum
DOI:
https://doi.org/10.18537/mskn.12.02.07Keywords:
Antifungal susceptibility, Trichophyton rubrum, Trichophyton mentagrophytesAbstract
The broth dilution method is considered the gold standard for the Minimum Inhibitory Concentration (MIC) determination of antimicrobials. The aim of this work was the evaluation in vitro of the antifungal susceptibility by standardization of the microdilution method of T. mentagrophytes and T. rubrum against the antifungals fluconazole, voriconazole, itraconazole, and terbinafine. The dilution methodology of the CLSI M61 guideline was used for the evaluation of dermatophytes from the LMC-FQ collection of the South Sierra of Ecuador. The MICs obtained for the strains of T. mentagrophytes against voriconazole are 0.03125 μg/mL, against itraconazole and terbinafine is 0.00781 μg/mL; for T. rubrum a range of 0.5-4 μg/mL was obtained against fluconazole, 0.01562 μg/mL against voriconazole and 0.00781 μg/mL against terbinafine. Standardization of the method was achieved by replicating the methodology described in the CLSI guidelines, obtaining reproducible and applicable results for a reference laboratory for mycological diagnosis. Resistant dermatophytes can be recognized, and anti-dermatophyte treatment strategies can be confirmed, improved or changed, and contribute to the epidemiological profile of the region, as well as for screening tests in search of new natural or synthetic compounds with antifungal activity.
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