Efficiency of ethanolic extract of eucalyptus (Eucalyptus globulus) in the control of Alternaria sp. in cabbage and potato crops

Authors

  • María Cázar Facultad de Ciencias Químicas, Universidad de Cuenca, Av. 12 de Abril s/n, Cuenca, Ecuador.
  • Paulina Villena Facultad de Ciencias Agropecuarias, Universidad de Cuenca, Campus Yanuncay.Programa Control de Plagas en Cultivos Hortícolas de la Región Centro Sur del Ecuador, Dirección de Investigación de la Universidad de Cuenca (DIUC), Av. 12 de Abril s/n, Cuenca, Ecuador.
  • Juan Parra Programa Control de Plagas en Cultivos Hortícolas de la Región Centro Sur del Ecuador, Dirección de Investigación de la Universidad de Cuenca (DIUC), Av. 12 de Abril s/n, Cuenca, Ecuador.Profesor Jubilado, Facultad de Ciencias Químicas, Universidad de Cuenca, Av. 12 de Abril s/n, Cuenca, Ecuador.
  • Virgilio Espinoza Programa Control de Plagas en Cultivos Hortícolas de la Región Centro Sur del Ecuador, Dirección de Investigación de la Universidad de Cuenca (DIUC), Av. 12 de Abril s/n, Cuenca, Ecuador.Profesor Jubilado, Facultad de Ciencias Químicas, Universidad de Cuenca, Av. 12 de Abril s/n, Cuenca, Ecuador.
  • Giovanni Larriva Facultad de Ciencias Químicas, Universidad de Cuenca, Av. 12 de Abril s/n, Cuenca, Ecuador.Programa Control de Plagas en Cultivos Hortícolas de la Región Centro Sur del Ecuador, Dirección de Investigación de la Universidad de Cuenca (DIUC), Av. 12 de Abril s/n, Cuenca, Ecuador.
  • Adriana Caldas Programa Control de Plagas en Cultivos Hortícolas de la Región Centro Sur del Ecuador, Dirección de Investigación de la Universidad de Cuenca (DIUC), Av. 12 de Abril s/n, Cuenca, Ecuador.

DOI:

https://doi.org/10.18537/mskn.05.01.03

Keywords:

eucalyptus globulus, biologic control, genus Alternaria, greenhouse experiment, field experiment

Abstract

The inhibitory effect of ethanolic extracts against Alternaria sp. in greenhouse cabbage cultivation (Oleracea brassica) and in-situ potato growing (Solanum tuberosum) was tested. Ethanolic extracts were prepared from Eucalyptus leaves (Eucalyptus globulus) with a Soxhlet extractor. Phytochemical analysis revealed the presence of considerable amounts of quinones, lactones and coumaranes, and small amounts of triterpenes and steroids. The experimental layout in the greenhouse cabbage cultivation consisted of three ethanol levels, one classical chemical treatment (Trizimand) and one control, ten cabbages per treatment, three repetitions, arranged in a randomized complete block design. The effect of three concentration levels of ethanol, one time sprayed on the leaves of the potato crop, was tested. The treatment plots, including a control plot, were randomized, each block 4 times repeated. In the greenhouse trial, the treatments with the effective dose increased by 50 and 25% displayed a comparable activity with the chemical treatment. Those treatments showed an increased effectiveness, compared with the effective dose at lab conditions. In the potato field essay, based on the incidence of pustules measured in the period of 65 to 85 days after planting, all treatments had an equal effect. It is believed that the observed overall minor and equal impact of the ethanol treatments on the potato crop is due to the high plot-to-plot variability and the more than normal wet conditions during the growing season.

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References

Amakura, Y., M. Yoshimura, N. Sugimoto, T. Yamazaki, T. Yoshida, 2009. Marker constituents of the natural antioxidant Eucalyptus leaf extract for the evaluation of food additives. Biosci., Biotechnol.,Biochem., 73(5), 1060-1065.

Aslam, A., M. Farah-Naz, R. Arshad, C. Rauf, 2010. In vitro antifungal activity of selected medicinal plant diffusates against Alternaria solani, Rhizoctonia solani and Macrophomina phaseolina. Pakistan J. Bot., 42(4), 2911-2919.

Batish, D., P. Harminder, K. Ravinder, K. Shalinder, 2008. Eucalyptus essential oil as a natural pesticide. For. Ecol. Manage., 256(12), 2166-2174.

Cazar, M., J. Parra, 2012. Extractos vegetales promisorios como biocontroladores de Botrytis cinerea y Alternaria sp. Biotecnología, 4, 29-32.

Corral Salvado, A., J. De la Paz Naranjo, E. Concepción Evseeva, R. Hernández Royero, D.L. López Rodríguez, 1997. Tamizaje, tecnología, control de calidad y farmacología del extracto fluido de Bouganvillea spetabilis Willd. Rev. Cubana Plant. Med., 2(2), 19-25.

Daintith, J. (Ed.), 2008. Dragendorff test. A dictionary of chemistry (6 ed.). Oxford University Press, UK, 592 pp.

Dayan, F., C. Cantrell, S. Duke, 2009. Natural products in crop protection. Bioorg. Med. Chem., 17(12), 4022-4034.

Duncan, D.B., 1955. Multiple range and multiple F tests. Biometrics, 11, 1-42.

Fehling, H., 1849. Die quantitative Bestimmung von Zucker und Stärkmehl mittelst Kupfervitriol. Ann. Chemie und Pharmacie, 72(1), 106-113.

Fisher, R.A., 1925. Statistical methods for research workers. Oliver & Boyd, Edinburgh, UK, 362 pp.

Gaybor A., C. Nieto, R. Velasteguí, 2006. TLC y plaguicidas: Impactos en los mercados y la agricultura ecuatoriana. Sistema de Investigación sobre la Problemática Agraria en el Ecuador (SIPAE), Quito, Ecuador, 130 pp.

Gullino, M., P. Leroux, C. Smith, 2000. Uses and challenges of novel compounds for plant disease control. Crop Prot., 19(1), 1-11.

Harish, S., D. Saravanakumar, R. Radjacommare, E.G. Ebenezar, K. Seetharaman, 2008. Use of plant extracts and biocontrol agents for the management of brown spot disease in rice. BioControl, 53(3), 555-567.

INHAMI (2012). Boletín climatológico anual. Año 2012. Descargado de http://www.serviciometeorologico.gob.ec/ en febrero de 2014.

Jabeen, K., A. Javaid, 2008. Antifungal activity of aqueous and organic extracts of allelopatic trees against Ascochyta rabiei. Allelopath. J., 22(1), 231-237.

Khajista, J., A. Javaid, 2008. Antifungal activity of aqueous and organic solvent extracts of allelopathic trees against Ascochyta rabiei. Allelopath. J., 22, 231-238.

Kotan, R., F. Dadasoglu, K. Karagoz, A. Cakir, H. Ozer, S. Kordali, R. Cakmakci, N. Dikbas, 2013. Antibacterial activity of the essential oil and extracts of Satureja hortensis against plant pathogenic bacteria and their potential use as seed disinfectants. Sci. Hortic., 153, 34 -41.

Koul, O., S. Walia, 2009. Comparing impacts of plant extracts and pure allelochemicals and implications for pest control. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 4(049), 1-30.

Mallikharjuna, P.B., L.N. Rajanna, Y.N. Seetharam, G.K. Sharanabasappa, 2007. Phytochemical studies of Strychnos potatorum L.F., a medicinal plant. Electron. J. Chem., 4(4), 510-518.

Mejía, L., E. Rojas, Z. Maynard, S. Van Bael, E. Arnold, P. Hebbar, G. Samuels, N. Robbins, A. Edward, 2008. Endophytic fungi as biocontrol agents of Theobroma cacao pathogens. BioControl, 46(1), 4-14.

Quintana, G.Y., A. Noboa, D. Alava, T. Yánez-N., 2008. Manual técnico para la gestión integral de plaguicidas. Programa de apoyo a la gestión descentralizada de los recursos naturales en las tres provincias del norte del Ecuador: Carchi, Esmeraldas e Imbabura (PRODERENA). Ministerio del Ambiente, Quito, Ecuador, 160 pp.

Raaijmakers, J.M., M. Vlami, J.T. De Souza, 2002. Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenhoek, 81(1-4), 537-547.

Spadaro, D., M. Gullino, 2005. Improving the efficacy of biocontrol agents against soilborne pathogens. Crop Prot., 24(7), 601-613.

Satish, S., M. Raghavendra, K. Raveesha, 2009. Antifungal potential of some plant extracts against Fusarium sp. Arch. Phytopathology Plant Protect., 42(7), 618-625.

Soxhlet, F., 1879. Die gewichtsanalytische Bestimmung des Milchfettes. Polytechnisches J., 232, 461.

Published

2014-06-25

How to Cite

Cázar, M., Villena, P., Parra, J., Espinoza, V., Larriva, G., & Caldas, A. (2014). Efficiency of ethanolic extract of eucalyptus (Eucalyptus globulus) in the control of Alternaria sp. in cabbage and potato crops. Maskana, 5(1), 33–41. https://doi.org/10.18537/mskn.05.01.03

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Section

Research articles