Evaluation of semi-solid culture systems and BIT for in vitro multiplication of Juglans neotropica
DOI:
https://doi.org/10.18537/mskn.08.01.09Keywords:
juglans neotropica, Temporary Immersion Bioreactors, Andean walnutAbstract
The purpose of this study was to evaluate two culture systems, semi-solid and temporary immersion in bioreactors (BIT), for the micropropagation of Juglans neotropica, using zygotic embryos. Additionally, in the BIT culture system also the effect of the frequency of immersion and forced aeration on the micropropagation was examined. The culture growth of the shoots was measured by the following characteristics: number and size of the shoots, number of leaves, and the wet and dry weight. No statistical differences were detected. In general, the BIT immersion system produced an equivalent number of plants of similar characteristics as the embryos propagated in the traditional semi-solid medium method. However, BITs produced more shoots, forced ventilation resulted in less hyperhydric shoots and the BIT system was considerable cheaper, resulting in an average production cost of $0.21 per plant versus $0.40 in the semi-solid propagation method.
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